Many translated example sentences containing “centro organizador” – English- Spanish centrosoma es el único centro organizador de microtubulos [ ]. tema el citoesqueleto. ‘red de filamentos proteicos con función esquelética que constituyen el “andamio” interno de la célula’. se encuentra en todas las. los microtúbulos están anclados por sus extremos negativos al centro organizador de microtúbulos y los extremos positivos se dirigen hacia la periferia ; durante.
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Country of ref document: Therefore, regulation of the immune response mirotubulos crucial in the treatment of the most important causes of morbidity and mortality in our society.
Sawing, JM, Nieto, M.
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TCR engagement proline-rich induces tyrosine micrtubulos Pyk2 translocation to the T cell-APC interface Pyk2 Independently of activity and in an immunoreceptor tyrosine-based activation motif-mediated fashion. The fibronectin fragment 80 kD FN80 was generously provided by Dr. Trapoxina B fue generosamente cedida por el Dr. After 6 hours of conjugate formation in the presence of superantigen E, production of IL-2 decreased drastically transfected with HDAC6-GFP cells, as this functional response was unaffected in cells that had been transfected with the mutant deficient Dentro deacetylase activity Fig.
Absence of dystrophin disrupts skeletal muscle signaling: Date of ref document: The percentage of mature synapse in J77 cells transfected with HDAC6 was two times lower than in cells transfected with the mutant deficient in deacetylation or in lymphocytes treated with TSA and or trapoxin B, or sodium butyrate modified it significantly Fig.
By contrast, levels of tubulin acetylation were not modified in the presence of anti-CD4 antibodies, anti-ICAM-3 or other irrelevant immunoglobulins Fig.
A method of identifying compounds according to claim 1 wherein the determination of the levels of tubulin acetylation of ii is performed by immunoblot techniques or Western blot with appropriate antibodies. And, more specifically to the field of methods of identifying novel therapeutic compounds and pharmaceutical compositions for treating diseases or disorders splenic immune system disorders.
Immunoblot analysis showed that levels of tubulin acetylation significantly increased in conjugates between T cells presenting cells in the presence of superantigen E but superantigen B Fig. In summary, the present invention is based on the discovery that microtubule acetylation is important to modulate the activation of T cells and is regulated by the HDAC6 protein, allowing define new therapeutic approaches for treating diseases splenic immune system disorders.
Basal levels of tubulin acetylation in T cells increased by treatment with such antibodies Fig. The following describes in detail the materials and methods used to perform the examples of the present invention. Nature; Furumai, R.
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The following examples serve to illustrate the invention and should not be considered as limiting the scope thereof. Nature82 ; Grakoui, A. Cytoskeletal polarization of T cells is regulated by an immunoreceptor tyrosine-based activation motif-dependent mechanism. Another particular object of the present invention is the method of identifying compounds of the invention in which the determination of a parameter related to the activation of T lymphocytes can be performed, for illustrative purposes and without limiting the scope of the present invention, by a technique belonging to the following group: In vivo destabilization of dynamic microtubules by HDAC6-mediated deacetylation.
Tyrosine-phosphorylated T cell receptor miceotubulos chain associates with the actin cytoskeleton upon activation of mature T lymphocytes.
It was found that tubulin acetylation in the cells increased by the action of the TSA Fig. Method of identifying compounds according to claim 1 wherein determining a parameter related to mixrotubulos activation of T cells ii is carried outamong other possible, by a technique belonging to the following group: The amount of acetylated microtubules was greater in specific superantigen conjugates that conjugates between cells of the same type or cell conjugates established in the absence of superantigen E Fig.
El tiempo transcurrido desde el contacto inicial en minutos se indica en la esquina superior izquierda de cada mcrotubulos.
Furthermore, it was carried out a quantitative analysis of conjugates containing J77 cells with CD3 clustered in the central region of intercellular contact over conjugates corresponding to three independent experiments. By contrast, acetylation levels not varied in the presence of superantigen B Fig. Activation of T cells requires antigen binding properly presented by molecules of the major histocompatibility complex antigen presenting cells.
Method of identifying and evaluating the activity regulating compounds HDAC6 protein on the activation of T lymphocytes characterized in that it comprises the following steps: Then the cell conjugates fixed, permeabilized and stained for acetylated tubulin. Tyrosine phosphorylated T-cell receptor zeta chain associates With the actin cytoskeleton upon activation of mature T lymphocytes.
TCR engagement induces proline-rich tyrosine kinase-2 Pyk2 translocation to the T cell-APC interface independently of Pyk2 activity and in an immunoreceptor tyrosine-based activation motif-mediated fashion. Three proteins define a class of human histone-deacetylases related to yeast Hdalp; Proc.