Disease. Gummy stem blight. Causes leaf spot, stem canker, black leaf and fruit rot; common name refers to the characteristic gummy exudate. Didymella bryoniae (Auersw.) Rehm [anamorph Phoma cucurbitacearum (Fr.) Sacc.] is the causative agent of gummy stem blight (GSB), a disease affecting. Gummy stem blight of cucurbits produces a variety of symptoms which are referred to as leaf spot, stem canker, vine wilt and black fruit rot. Lesions on leaves.

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Muskmelon seedlings were inoculated with the two genotypes of D. Stems may be infected by D. In addition, although the developed LAMP assay showed high sensitivity and broad-spectrum detection of D.

Rapid, specific, simple, in-field detection of Xanthomonas campestris pathovar musacearum by loop-mediated isothermal amplification. Didymella bryoniae Fuckel Rehm, Leaf spots of gummy stem blight often are round bottle gourd, Lagenaria siceraria. Water-soaked lesions developed around inoculation sites and microscopy of infected tissue revealed pycnidia with conidia as described above.


The occurrence of GSB disease in the greenhouse and in the field may didymflla from transplantation of latently infected source seedlings, or contamination by didyemlla pathogen may occur as a result of inappropriate pruning of unhealthy plants Keinath,; Ling et al.

Didymella bryoniae

Detection of cucumber mosaic virus isolates from banana by one-step reverse transcription loop-mediated isothermal amplification. In latter periods of growth, the mycelium is an olive to dark green or black color [7]. Highlights of the Didymellaceae: Black rot also affects watermelon when foliar blight is severe The field is not protected in any way. Incubate fresh material to allow production didtmella spores within leaf, petiole, and fruit lesions. Go to distribution map On watermelon leaves, the shape of anthracnose spots is distinctly angular and irregular, while leaf spots of gummy stem blight generally are round.

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First report of gummy stem blight, caused by Didymella bryoniaeon watermelon transplants in California. Cankers may form on crowns, main stems, or vines of melons and other hosts Topical Meetings and Workshops. Each product was confirmed by 2.


Compared with the result of Ling et al. To control GSB of cucurbit crops, early crops with latent infection must be accurately detected and removed.

Didymella bryoniae

BoxArusha, Tanzania Go to article: The bicellular ascospores have a diagnostic shape: Walker, Mycosphaerella melonis Pass. To produce inoculum, isolates of D.

In our study, D. Young pycnidia are tan with a dark ring of cells around the ostiole Fig. One reason for this could be that in the present study we identified a suitable loop-backward primer and used the primer to accelerate the reaction.

With these advantages, the LAMP method has been widely used for detection of plant pathogens, such as viruses Hadersdorfer et diddymella.

As leaf spots expand, they coalesce, which results in leaf blighting Fig. Didymella bryoniae is a pathogenic fungus that causes gummy stem blight GSB in Cucurbitaceae crops e. All isolates infected both hosts. With age these spots darken to brown and black [1,2].

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